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  • SAGA – Genetic Analysss Service for Agriculture


    This is a high-throughput genotyping platform capable of simultaneously developing genomic profiles for thousands of DNA samples. The genetic characterization is based on the production of tens or even hundreds of thousands of molecular markers. The platform uses the DArTseq method, developed by Diversity Arrays Technology (DArT), and has the capacity to identify two types of polymorphisms, In silico DArT (PAV – Presence/Absence of Variation) and SNPs (Single Nucleotide Polymorphism).

    Product details and features

    1. The customer must supply the DNA for analysis as follows: 1) The preferred format is 94 samples supplied in sealed 96-well microliter plates, leaving wells G12 and H12 empty; 2) DNA suitable for restriction enzyme digestion and ligation; 3) each sample about 30ul of DNA in TE or other aqueous buffer at about 50-100 ng/ul; 4) shipped to us following the shipping instructions.
    2. To avoid leaks and cross contamination of samples during shipment we strongly recommend: 1) Fully skirted, V-shape bottom 96 well PCR plates Twin-Tec form Eppendorf; 2) Strips of 8 clear FLAT caps from Sarstedt ref 65.1998.002; 3) Seal well!; 4) Package in a rigid box
    3. SAGA also offers DNA extraction service, with additional cost. The client must send seed of each material, which will be planted to extract DNA from leaves of seedlings in greenhouse. The seedlings will be destroyed after taking the leaf sample for DNA extraction.


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  • Comments

    The genotyping service includes advice on the interpretation of the genotypic data produced.

     

    Availability

    Currently available to internal and external services, for any agronomically important crop.



    Primary Users

    Researchers, breeders and students involved in all areas of scientific research that include the use of large sets of molecular data for use in breeding programs, genetic diversity studies, or the conservation of biodiversity.

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